RNAsqe of Drosophila simulans: ovaries - SRA

SRX24778837: RNAsqe of Drosophila simulans: ovaries
1 ILLUMINA (Illumina NovaSeq 6000) run: 15.3M spots, 4.6G bases, 1.4Gb downloads

Design: RNA-sequencing libraries were generated using the NEBNext Poly(A) mRNA Magnetic Isolation Module followed by the NEBNext Ultra II Directional RNA Library Kit for Illumina. Briefly, adult females were dissected in ice-cold PBS and 40-50 ovaries were flash-frozen and stored at -80C until further processing. Total RNA was collected from the Lexogen TraPR Small RNA isolation columns using TRIzol LS (after small RNA isolation), and subsequently purified with the addition of chloroform and isopropanol. After purification, 2.5g of RNA was measured by Qubit RNA HS Assay kit (Thermo Scientific) and used for poly(A) isolation and library preparation. Libraries were amplified for 8 cycles, and quality checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific). The multiplexed libraries were sequenced as 150bp paired-end reads on the Illumina Novaseq 6000 SP.

Submitted by: University of Cambridge

Study: Drosophila species HiC and RNA sequencing

PRJNA1119277 • SRP511279 • All experiments • All runs

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We generated HiC data from whole female flies for 22 Drosophila species, for which ONT-sequencing-based genome assemblies are available, to scaffold these assemblies in order to achieve chromosome-level genome quality, together with 8 species with publicly available HiC data. We further produced RNA sequencing data from ovaries for these 30 species to annotate the scaffolded genome assemblies.

Sample:

SAMN41649409 • SRS21497319 • All experiments • All runs

Organism: Drosophila simulans

Library:

Name: SLX-20415.NEBNext22

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 15.3M spots, 4.6G bases, 1.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR29261107	15,345,055	4.6G	1.4Gb	2024-06-24

ID:: 33104175

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